Isolation of Mitochondria from Tissue Culture Cells
نویسندگان
چکیده
منابع مشابه
Isolation of mitochondria from tissue culture cells.
The number of mitochondria per cell varies substantially from cell line to cell line. For example, human HeLa cells contain at least twice as many mitochondria as smaller mouse L cells. This protocol starts with a washed cell pellet of 1-2 mL derived from ∼10⁹ cells grown in culture. The cells are swollen in a hypotonic buffer and ruptured with a Dounce or Potter-Elvehjem homogenizer using a ti...
متن کاملIsolation of Adipose Tissue Stem Cells with Organ Culture Method
Background Isolation of adipose tissue mesenchymal stem cells (MSCs) with current enzymatic methods has some limitations. For example, it is costly and time-consuming and results in a heterogeneous cell population that making compromise proliferation and differentiation of MSCs. Also, it is accompanied with the increased risk of culture contaminations because of several handling steps.In this...
متن کاملP9: Isolation and Culture of Adult Microglia Cells Derived from Epileptic Human Brain Tissue
لطفاً به چکیده انگلیسی مراجعه شود.
متن کاملTransferring isolated mitochondria into tissue culture cells
We have developed a new method for introducing large numbers of isolated mitochondria into tissue culture cells. Direct microinjection of mitochondria into typical mammalian cells has been found to be impractical due to the large size of mitochondria relative to microinjection needles. To circumvent this problem, we inject isolated mitochondria through appropriately sized microinjection needles...
متن کاملIsolated Mitochondria with Tissue Culture Cells
Experiments were undertaken to study the effect of introducing isolated mammalian cell mito chondria into tissue culture cells. The D N A of isolated mitochondria was labeled in vitro with 3 Hthymidine. Incorporation of 3H-thymidine into mitochondrial D N A was increased tenfold by the addition of bovine serum albumin and sucrose to the assay. Labeled HeLa cell mitochondria were fused with WI38...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2014
ISSN: 1559-6095
DOI: 10.1101/pdb.prot080002